Abstract: Based on connotation, origin and development of the eucalyptus culture in Australian，this paper introduces the effect of eucalyptus culture on economic and ecological environment. By contracting the difference of eucalyptus culture in spiritual level in China and in Australia, the effect of Chinese eucalyptus culture influenced by traditional culture and social factors is revealed. Analyzing literatures about eucalyptus culture in Chinese National Knowledge Infrastructure (CNKI), the authors find that the regional economic disparities result in the difference of research on the Eucalyptus culture. The imbalance of research direction on the eucalyptus culture correctly can benefit the constructions of spiritual civilization, economy and ecology in China.