Abstract: Zuoyinyuan was a private garden built by WANG Tingne in the 28th year (1600 A.D.) of Wanli’s reign of the Ming Dynasty. It covered a large area and had many scenic spots. Today, there are still some uncertainties in the owner and the site of the garden, that need to be clarified. In this paper, we analyzed three newly found books, Concise Family Tree of Wang, Yingchuan Yueyintang Family Tree of Wang, and Huancuitang Huagunji. It is found that the family members and their descendants of WANG Tingne were arranged clearly in the books. At the same time, WANG Tingne has been proved to be WANG Shiqi’ natural son and not adopted son, which is a correction to the long-time misunderstanding of WANG Tingne’s identity. New evidences have been given on the site of Zuoyinyuan, which further proved that the Zuoyinyuan was sited at Wangcun Village of Wan’an Town, Xiuning County, Anhui Province (today’s Chenkeng Village, Xintan Town of the Huangshan Economic Development Zone).