Abstract: Under the promotion of the rural revitalization strategy, the whole society invests more and more in rural tourism, which has promoted the development of rural industry and the prosperity of farmers. Taking Zhejiang Province as an example, we selected the panel data of 569 continuous sample villages from 2014 to 2018, and studied the impact of rural tourism investment on the economic benefit of rural tourism by considering factors such as resource endowment, rural tourism infrastructure conditions, regional economic development level, etc. LM test, F test and Hausman test were used to identify the panel data model type, and Stata13.0 was adopted to establish the random effects model. The results show that rural tourism investment has a significantly positive effect on the total income of rural tourism operation. For every 1% increase in rural tourism investment, the total income of rural tourism operation will increase by 0.24%, suggesting that government tourism investment plays an important role in improving rural economic benefits. Finally, this paper puts forward policy suggestions on how to improve the effectiveness of tourism investment.