Abstract: Ecosystem hydrological regulation services have an important impact on human well-being. In this paper, InVEST water yield model and Support Vector Regression (SVR) model are used to evaluate and predict the hydrological regulation value of ecosystem in Qinghai area of the Qilian Mountain National Park. The results show as follows: ① Grassland plays an important role in hydrological regulation in Qinghai area of the Qilian Mountain National Park. In 2020, The hydrological regulation monetary value of grassland was RMB 3 129 million yuan, accounted for 60.06% of the total value. ② Although the area of forestland is not large, it plays an obvious role in ensuring ecological security. The hydrological regulation monetary value of forestland in 2020 was RMB 725 million yuan, accounting for 13.92% of the total hydrological regulation value. ③ From 2010 to 2020, the amount of hydrological regulation of different ecosystems in Qinghai area of the Qilian Mountain National Park increased by 2.24% annually, and the monetary value of hydrological regulation increased by about 0.97% annually, which was less than the annual growth rate of the physical value, mainly due to the decline of hydrological regulation monetary value of some ecosystems. ④ Compared with 2020, the amount of hydrological regulation physical and monetary value will increase by 490 million tons and 3.86% in 2030.