Abstract: Based on the related concepts of eucalyptus culture and Lingnan culture, this paper studied people's awareness and cognition of eucalyptus and eucalyptus culture by the method of stratified sampling, analyzed what led to differences of people's cognition of eucalyptus and eucalyptus culture, summarized the regional differences of eucalyptus culture in China, and reflected the development state of eucalyptus culture in Lingnan area. By researching the relevant literature, the authors expounded the correlation of eucalyptus culture and Lingnan culture, revealing that eucalyptus culture promoted the development and changes of Lingnan culture as well as the rationality and importance of planting eucalyptus. This paper also fully unearthed the spiritual content of eucalyptus culture, which is of great significance to the industrial development of eucalyptus, the building of spiritual civilization and the protection of ecological environment in Lingnan area. Proper understanding, development and promotion of eucalyptus culture can contribute to the development of Lingnan area and Lingnan culture.