Abstract: The cultivating subjects of ecological culture include the state, society and citizen. The state is the main driving body of the cultivating ecological culture, mainly provides direction framework, norms and institutional guarantee, and funding support for the cultivation of ecological culture. Society as the gathering of a whole is the major part of main body of cultivating ecological culture; the social units such as enterprises, communities, and non-governmental environmental organizations play different roles, complement each other, and co-cultivate, thus ultimately the unity of ecological society and ecological culture achieves. Citizen is the most basic subject in the cultivation of ecological culture, and citizen practice is the endless source of ecological culture. On the one hand, citizen practice is the "the last kilometer" during the cultivation of ecological culture, and the state-led and social cultivation are finally implemented in citizen practice; on the other hand, citizen practice constantly refine and enrich the content of ecological culture. The main way for citizens to cultivate ecological culture is to choose the green consumption and green life, and carry out public discussion and environmental rights protection, etc., i. e., the ultimate result is ecology from individual to whole human beings.